Experimental design for gene expression microarrays
نویسندگان
چکیده
منابع مشابه
Experimental design for gene expression microarrays.
We examine experimental design issues arising with gene expression microarray technology. Microarray experiments have multiple sources of variation, and experimental plans should ensure that effects of interest are not confounded with ancillary effects. A commonly used design is shown to violate this principle and to be generally inefficient. We explore the connection between microarray designs...
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The oligonucleotide probes on microarrays for gene expression are carefully designed so that their thermodynamic properties are consistent, in order that any bias due to differential efficiencies of hybridisation might be minimized. However there are numerous other constraints such as the need to match (and be specific to) a gene, as well as possibly having a fixed probe length. Thus a perfect ...
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ژورنال
عنوان ژورنال: Biostatistics
سال: 2001
ISSN: 1465-4644,1468-4357
DOI: 10.1093/biostatistics/2.2.183